Search result for 'flower '.
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N16312
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Name: hapless 6 |
Price:
£11.00
|
Donor
- Brown University Mark Johnson
|
Locus
|
Stock type: individual line
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Material type: seed
|
|
Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
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Phenotype
Distorted segregation, reduced transmission through the male gametophyte, does not appreciably affect the female gametophyte; completely disrupted male functions, siring no BastaR progeny when crossed to wild type; short pollen tube growth, failing to exit the style.
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N16313
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Name: hapless 7 |
Price:
£11.00
|
Donor
- Brown University Mark Johnson
|
Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
|
Phenotype
Distorted segregation, male gametophyte and female gametophyte are severely affected; short pollen tube growth, failing to exit the style.
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N16314
|
Name: hapless 8 |
Price:
£11.00
|
Donor
- Brown University Mark Johnson
|
Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
|
Phenotype
Distorted segregation, affecting the male gametophyte and the female gametophyte, decreasing, but not eliminating, the function of both gametophytes; short pollen tube growth, failing to exit the style.
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N16315
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Name: hapless 9 |
Price:
£11.00
|
Donor
- Brown University Mark Johnson
|
Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
|
Phenotype
Distorted segregation, reduced transmission through the male gametophyte, does not appreciably affect the female gametophyte; strong, but not complete impact on pollen function; short pollen tube growth, failing to exit the style.
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N16316
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Name: hapless 10 |
Price:
£11.00
|
Donor
- Brown University Mark Johnson
|
Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
|
Phenotype
Distorted segregation, reduced transmission through the male gametophyte, does not appreciably affect the female gametophyte; pollen tube growth in ovary is short, but pollen tubes target the ovules they reach.
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N16317
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Name: hapless 11 |
Price:
£11.00
|
Donor
- Brown University Mark Johnson
|
Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
|
Phenotype
Distorted segregation, reduced transmission through the male gametophyte, does not appreciably affect the female gametophyte; pollen tube growth path appears normal, yet tubes fail to enter the micropyle.
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N16318
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Name: hapless 13 |
Price:
£11.00
|
Donor
- Brown University Mark Johnson
|
Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
|
Phenotype
Distorted segregation, reduced transmission through the male gametophyte, does not appreciably affect the female gametophyte; completely disrupted male functions, siring no BastaR progeny when crossed to wild type; short pollen tube growth, failing to exit the style.
|
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N16319
|
Name: hapless 14 |
Price:
£11.00
|
Donor
- Brown University Mark Johnson
|
Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
|
Phenotype
Distorted segregation, reduced transmission through the male gametophyte, does not appreciably affect the female gametophyte; pollen tube growth in ovary is short, but pollen tubes target the ovules they reach.
|
|
N16320
|
Name: hapless 15 |
Price:
£11.00
|
Donor
- Brown University Mark Johnson
|
Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
|
Phenotype
Distorted segregation, reduced transmission through the male gametophyte, does not appreciably affect the female gametophyte; strong, but not complete impact on pollen function; short pollen tube growth, failing to exit the style.
|
|
N16321
|
Name: hapless 16 |
Price:
£11.00
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Donor
- Brown University Mark Johnson
|
Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
|
Phenotype
Distorted segregation, reduced transmission through the male gametophyte, does not appreciably affect the female gametophyte; completely disrupted male functions, siring no BastaR progeny when crossed to wild type; disrupted pollen grain development.
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